The 16S rRNA gene of Streptomyces lividans TK64 contains internal promoters

Su Chih Chang, Wen Chin Yang, Yan-Hwa Wu Lee*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

A 632-bp Sau3AI fragment of Streptomyces lividans TK64 genome was found to confer promoter activity in Streptomyces and Escherichia coli. This fragment showed almost identical sequence (97.8%) to the S. coelicolor 16S rRNA segment encompassing from nucleotide 706 to 1337 region. The transcription start points of this fragment were identified by the primer extension method. Analysis of the nucleotide sequence upstream the transcription start points revealed two putative E. coli-like promoters resided within this fragment. The occurrence of internal promoters active in Streptomyces and E. coli was also confirmed in the 16S rRNA gene of rrnE operon from TK64.

Original languageEnglish
Pages (from-to)219-222
Number of pages4
JournalBBA - Gene Structure and Expression
Volume1129
Issue number2
DOIs
StatePublished - 6 Jan 1992

Keywords

  • (E. coli-like promoter)
  • (Streptomyces)
  • 16S
  • DNA sequence
  • Internal promoter
  • Primer extension
  • rrn operon
  • rRNA gene

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