Targeted RGD nanoparticles for highly sensitive in vivo integrin receptor imaging

Ren Yen Lin, Kasala Dayananda, Ting Jung Chen, Chiao Yun Chen, Gin Chung Liu, Kun Liang Lin, Yun-Ming Wang*

*Corresponding author for this work

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

A new magnetic resonance imaging (MRI) contrast bearing RGD peptide is reported. In this study, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles with various sizes were prepared. Particles sizes between 6 and 13nm were tuned by varying the stirring rate. Remarkable negative contrast was observed because USPIO nanoparticles (13.1±2.1nm) generated high transversal relaxivity r2 (188±3m m-1s-1) and saturation magnetization (94emug-1 Fe). The USPIO nanoparticles were coated with PDA [2-(pyridyldithio)-ethylamine; PDA nanoparticles] containing functional polymer, which can be readily synthesized by Michael addition. The PDA nanoparticles were conjugated with RGD peptide (RGD nanoparticles) for targeting the specific site. The target specificity and high relaxivity allowed RGD nanoparticles to differentiate the expression level of integrin receptor on several cell lines and tumors (MCF-7, A-549, HT-29 and HT-1080) by in vitro and in vivo MR imaging. Importantly, a remarkable negative contrast (-51.3±6.7%) was observed for in vivo MR imaging of MCF-7 tumor. This result implies that the RGD nanoparticles that greatly enhance the MR imaging are highly sensitive for early stage tumor detection.

Original languageEnglish
Pages (from-to)7-18
Number of pages12
JournalContrast Media and Molecular Imaging
Volume7
Issue number1
DOIs
StatePublished - 1 Jan 2012

Keywords

  • Cell
  • Fluorescence
  • Magnetic resonance imaging (MRI)
  • Peptide
  • Superparamagnetic nanoparticles (SPIO)

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