Systems-level modeling with molecular resolution elucidates the rate-limiting mechanisms of cellulose decomposition by cellobiohydrolases

Barry Z. Shang, Rakwoo Chang, Jhih-Wei Chu*

*Corresponding author for this work

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Background:Cellobiohydrolase enzymes processively degrade crystalline cellulose into free sugar molecules. Results:A spatially resolved kinetic model has been developed to understand the effects of interfacial confinement on cellobiohydrolase activity. Conclusion:Cellobiohydrolase activity is limited by slow rates of complexation with cellulose and traffic jamming among enzymes on the substrate. Significance:Identifying kinetic effects imposed by interfacial confinement is crucial for understanding and engineering cellulose bioconversion.

Original languageEnglish
Pages (from-to)29081-29089
Number of pages9
JournalJournal of Biological Chemistry
Volume288
Issue number40
DOIs
StatePublished - 4 Oct 2013

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