Synthesis of activity probes for β-xylosidase

Lee Chiang Lo*, Chi Yuan Chu, Fang An Lin, Yen Ru Pan, Yaw-Kuen Li, Jing Jer Lin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Due to their industrial interest and significance, developing a rapid method for screening β-xylosidase activities would certainly facilitate the research and improve production in xylan-related industries especially in the pulp and paper industries. Here we report the synthesis of two activity probes, LCL-6X and -12X, for β-xylosidase. They both carry a β-xylopyranosyl recognition head, which is linked to a latent trapping device consisting of a 2-fluoromethylphenoxyl group and a biotin reporter group. They differ only in the length of their hydrophobic linker. A model study using a β-xylosidase from T. koningii demonstrates that both probes could successfully label the target hydrolase and give rise to biotinylated proteins. They could thus potentially become a powerful tool in screening β-xylosidase from microbial sources.

Original languageEnglish
Pages (from-to)479-488
Number of pages10
JournalJournal of the Chinese Chemical Society
Volume53
Issue number2
DOIs
StatePublished - 1 Jan 2006

Keywords

  • Activity probe
  • Biotin
  • Labeling
  • Mechanism-based
  • Proteome
  • Quinone methide
  • Xylan
  • Xylosidases

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