Synergistic effects of plasma-activated medium and chemotherapeutic drugs in cancer treatment

Chao Yu Chen, Yun-Chien Cheng, Yi Jing Cheng

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Chemotherapy is an important treatment method for metastatic cancer, but the drug-uptake efficiency of cancer cells needs to be enhanced in order to diminish the side effects of chemotherapeutic drugs and improve survival. The use of a nonequilibrium low-temperature atmospheric-pressure plasma jet (APPJ) has been demonstrated to exert selective effects in cancer therapy and to be able to enhance the uptake of molecules by cells, which makes an APPJ a good candidate adjuvant in combination chemotherapy. This study estimated the effects of direct helium-based APPJ (He-APPJ) exposure (DE) and He-APPJ-activated RPMI medium (PAM) on cell viability and migration. Both of these treatments decreased cell viability and inhibited cell migration, but to different degrees in different cell types. The use of PAM as a culture medium resulted in the dialkylcarbocyanine (DiI) fluorescent dye entering the cells more efficiently. PAM was combined with the anticancer drug doxorubicin (Doxo) to treat human heptocellular carcinoma HepG2 cells and human adenocarcinomic alveolar basal epithelial A549 cells. The results showed that the synergistic effects of combined PAM and Doxo treatment resulted in stronger lethality in cancer cells than did PAM or Doxo treatment alone. To sum up, PAM has potential as an adjuvant in combination with other drugs to improve curative cancer therapies.

Original languageEnglish
Article number13LT01
JournalJournal of Physics D: Applied Physics
Volume51
Issue number13
DOIs
StatePublished - 7 Mar 2018

Keywords

  • cancer cell
  • chemotherapeutic drug
  • HO
  • helium-based low-temperature atmospheric-pressure plasma
  • molecule uptake
  • plasma-activated medium

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