Synergistic effect of prostaglandin F2α and cyclic AMP on glucose transport in 3T3-L1 adipocytes

Guang-Yuh Chiou, Jim C. Fong*

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The combined effect of prostaglandin F2α (PGF2α) and cAMP on glucose transport in 3T3-L1 adipocytes was examined. In cells pretreated with PGF2α and 8-bromp cAMP for 8 h, a synergy between these two agents on glucose uptake was found. Insulin-stimulated glucose transport, on the other hand, was only slightly affected. The synergistic effect of these two agents was suppressed in the presence of cycloheximide and actinomycin D. In concord, immunoblot and Northern blot analyses revealed that GLUT1 protein and mRNA levels were both increased in cells pretreated with both PGF2α and 8-bromo cAMP, greater than the additive effect of each agent alone. The synergistic action of PGF2α with 8-bromo cAMP to enhance glucose transport was inhibited by GF109203X, a selective protein kinase C (PKC) inhibitor. In addition, in cells depleted of diacylglycerol-sensitive PKC by prolonged treatment with 4β-phorbol 12β-myristate 13α-acetate, a PKC activator, the synergistic effects of PGF2α and 8-bromo cAMP on glucose transport and GLUT1 mRNA accumulation were both abolished. Taken together, these results indicate that PGF2α may act with cAMP in a synergistic way to increase glucose transport, probably through enhanced GLUT1 expression by a PKC-dependent mechanism.

Original languageEnglish
Pages (from-to)627-634
Number of pages8
JournalJournal of Cellular Biochemistry
Volume94
Issue number3
DOIs
StatePublished - 15 Feb 2005

Keywords

  • 3T3-L1 adipocytes
  • Cyclic AMP
  • Glucose transport
  • GLUT1
  • Prostaglandin F2α
  • Protein kinase C

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