Surface plasmon resonance phase-shift interferometry: Real-time DNA microarray hybridization analysis

Shean-Jen Chen*, Yuan Deng Su, Feng Ming Hsiu, Chia Yuan Tsou, Yi Kuang Chen

*Corresponding author for this work

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Surface plasmon resonance (SPR) phase-shift interferometry, (PSI) is a novel technique which combines SPR and modified Machzehnder PSI to measure the spatial phase variation caused by biomolecular interactions upon a sensing chip. The SPR-PSI imaging system offers high resolution and high-throughout screening capabilities for microarray DNA hybridization without the need for additional labeling, and provides valuable quantitative information. The SPR-PSI imaging system has an enhanced detection limit of 2.5 × 10T7 refraction index change, a long-term phase stability of π/100 in 30 min, and a spatial phase resolution of π/300 with 100×100 μm2 detection area. This study successfully demonstrates the label-free observation of 15-mer DNA microarray.

Original languageEnglish
Article number034005
JournalJournal of Biomedical Optics
Volume10
Issue number3
DOIs
StatePublished - 1 May 2005

Keywords

  • DNA hybridization
  • Imaging system
  • Microarray
  • Phase-shifting interferometry
  • Surface plasmon resonance

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