Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging

R. Y. He, K. C. Cho, N. S. Chang, Y. D. Su, Shean-Jen Chen*

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

1 Scopus citations

Abstract

A surface plasmon-enhanced two-photon total-internal-reflection fluorescence (TIRF) microscope has been developed to provide the fluorescent images of living cell membranes. The proposed microscope with the helps of surface plasmons (SPs) not only provides brighter fluorescent images based on the mechanism of local electromagnetic field enhancement, but also reduces photobleaching due to having shorter fluorophore lifetime. In comparison with one-photon TIRF, the two-photon TIRF can achieve higher signal-to-noise ratio cell membrane imaging due its smaller excitation volume and lower scattering. Combining with the SP enhancement and two-photon excitation TIRF, the microscope has demonstrated the brighter and more contrast fluorescence membrane images of living monkey kidney COS-7 fibroblasts transfected with an EYFP-MEM or EGFP-WOX1 construct.

Original languageEnglish
Article number71831L
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7183
DOIs
StatePublished - 1 Jun 2009
EventMultiphoton Microscopy in the Biomedical Sciences IX - San Jose, CA, United States
Duration: 25 Jan 200927 Jan 2009

Keywords

  • Fluorescence microscopy
  • Living cell membrane
  • Surface plasmon
  • Total internal reflection
  • Two-photon excitation

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