Superoxide dismutase activities of spermatozoa and seminal plasma are not correlated with male infertility

Yao Yuan Hsieh, Yu Ling Sun, Chi Chen Chang, Yi San Lee, Horng Der Tsai, Chih-Sheng Lin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Abnormal reactive oxygen species (ROS) production is associated with defective sperm function. Superoxide dismutase (SOD) is related with the scavenging of seminal ROS. We aimed to determine the effect of SOD activities of spermatozoa and seminal plasma on sperm quality. Semen samples from infertile couples who consented to the analyses were divided into two groups: 1) normospermia (n = 20); and 2) oligoasthenozoospermia (n = 31). The SOD activities of the spermatozoa and seminal plasma were measured by determining the inhibition of pyrogallol autoxidation. The SOD activities of spermatozoa and seminal plasma in both groups were compared. The relationships between the SOD activities and the sperm qualities were determined. We noted that SOD activities of sperm/seminal plasma in both groups were nonsignificantly different (group 1 vs. 2 = 0.77 ± 0.33/0.84 ± 0.40 U/mg protein for sperm, and 0.66 ± and 0.36/0.83 ± 0.47 U/ml for seminal plasma). SOD activities of sperm/seminal plasma were positively but nonsignificantly correlated with the sperm motility (SOD of sperm = 0.0008 × motility + 0.67; SOD of seminal plasma = 0.0006 × motility + 0.81) and concentration (SOD of sperm = 0.0006 × concentration + 0.67; SOD of seminal plasma = 0.0021 × concentration + 0.73). We concluded that SOD activities of sperm and seminal plasma were nonsignificantly correlated with the seminal quality. It appears that the SOD survey is not a useful tool for determining sperm fertilization potential.

Original languageEnglish
Pages (from-to)127-131
Number of pages5
JournalJournal of Clinical Laboratory Analysis
Volume16
Issue number3
DOIs
StatePublished - 20 May 2002

Keywords

  • Antioxidant
  • Male infertility
  • Oligoasthenozoospermia
  • Reactive oxygen species
  • Sperm
  • Superoxide dismutase

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