Submicron-grooved culture surface extends myotube length by forming parallel and elongated motif

Chen Ching Yuan, Kuang Jen Ma, Kuei Chi Li, Hsi Hsin Chien, Huai En Lu, Ching-Ping Tseng, Shiaw Min Hwang

Research output: Contribution to journalArticle

6 Scopus citations


During skeletal muscle development, correct cellular orientation is vital to generate desired longitudinal contraction for functional muscle fibres. In this reported study, submicron-imprint lithography was used to generate submicron-grooved surfaces on polystyrene plates to induce striated myotubes in vitro. Mouse muscle myoblast cells cultured on a submicron-grooved surface migrated faster in a directionally uniform fashion; in comparison, cells cultured on a flat surface grew and migrated slower in indiscriminate directions. Subsequent maturation of the myoblast cells formed along the submicron-groove surface resulted in a tandem of parallel myotubes that were both longer and greater in circumference than in the case of the flat surface. In a functional test, the co-culture submicron-groove-grown myotubes with neurotransmitter secreting cells further demonstrated contraction abilities, suggesting submicron-groove-guided growth served to enhance myotube formation while retaining striated motifs and physiological functionality for muscle tissue engineering.

Original languageEnglish
Pages (from-to)440-444
Number of pages5
JournalMicro and Nano Letters
Issue number8
StatePublished - 9 Sep 2013

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