TY - JOUR
T1 - Study of the line optical tweezers characteristics using a novel method and establishing a model for cell sorting
AU - Lin, Ho Chien
AU - Hsu, Long
PY - 2009/7/1
Y1 - 2009/7/1
N2 - Optical tweezers have become a powerful tool in cellular and molecule biology. Line optical tweezers enhanced its function in cell sorting. This study presents the line trap model, based on the ray-optics model, and demonstrates its accuracy for the line optical tweezers. The line optical tweezers system is established to produce the optical intensity distribution of a line pattern and to trap the micro-sized beads. The main parameter, optical intensity distribution, is used to calculate the trapping force distribution in the model. The two forces, trapping force and water dragging force, and the equation of motion is used to simulate the trajectory of micro-sized beads as they pass through the line pattern in flowing water in the microchannel. The trajectory is analyzed to determine the effective separation distance between the microsized beads or cells. The method will be applied in biological and medical detection.
AB - Optical tweezers have become a powerful tool in cellular and molecule biology. Line optical tweezers enhanced its function in cell sorting. This study presents the line trap model, based on the ray-optics model, and demonstrates its accuracy for the line optical tweezers. The line optical tweezers system is established to produce the optical intensity distribution of a line pattern and to trap the micro-sized beads. The main parameter, optical intensity distribution, is used to calculate the trapping force distribution in the model. The two forces, trapping force and water dragging force, and the equation of motion is used to simulate the trajectory of micro-sized beads as they pass through the line pattern in flowing water in the microchannel. The trajectory is analyzed to determine the effective separation distance between the microsized beads or cells. The method will be applied in biological and medical detection.
UR - http://www.scopus.com/inward/record.url?scp=72049120931&partnerID=8YFLogxK
U2 - 10.1143/JJAP.48.072502
DO - 10.1143/JJAP.48.072502
M3 - Article
AN - SCOPUS:72049120931
VL - 48
JO - Japanese Journal of Applied Physics
JF - Japanese Journal of Applied Physics
SN - 0021-4922
IS - 7 PART 1
M1 - 072502
ER -