Study of cell adhesion and migration by using a plasmon-enhanced total internal reflection fluorescence microscope

R. Y. He, K. C. Chiu, Y. D. Su, K. L. Chang, Shean-Jen Chen*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

Total internal reflection fluorescence microscopy (TIRFM) induces the evanescent field from an incident light with an incident angle greater than the critical angle selectively to excite fluorescent molecules on or near a surface. The TIRFM not only provides enhanced understanding of cellular function but also improves signal-to-noise ratio of detecting signal in real time. However, fluorescent emission need to be increased when a dynamic biomolecular image is requested at the frame rate of greater than 100 frames/s. Therefore, the fluorescent signal is enhanced via surface plasmons to match the requirements of better efficiency and larger quantity. In this study, a plasmon-enhanced TIRFM whose operation is based on the electromagnetic field enhancement via surface and particle plasmon effects offered by a nano-scalar silver thin film and particles has been presented. The developed microscopy has been successfully used in the real-time observation of the enhanced fluorescence from the thrombomodulin protein of a living cell membrane. The simulated and experimental results demonstrate that the plasmon-enhanced TIRFM can provide brighter living cell images through surface plasmon enhanced fluorescence.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
DOIs
StatePublished - 8 May 2006
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV - San Jose, CA, United States
Duration: 23 Jan 200625 Jan 2006

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6088
ISSN (Print)1605-7422

Conference

ConferenceImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV
CountryUnited States
CitySan Jose, CA
Period23/01/0625/01/06

Keywords

  • Cell adhesion
  • Evanescence wave
  • Plasmonic effect
  • Surface plasmon resonance
  • Total internal reflection fluorescence microscope

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