Structural stability of heterologous genes cloned in Streptomyces plasmid pIJ702

Yan-Hwa Wu Lee*, Zee Yuan Tzecheng, Shiang Ching Wang, Wen Ling Cheng, Carton W. Chen

*Corresponding author for this work

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A recombinant plasmid pWCL1 containing Streptomyces plasmid pIJ702, E. coli plasmid pUC12, and hepatitis B viral surface antigen (HBsAg) gene was stably maintained in E. coli, but exhibited structural instability in S. lividans 1326. The deletions were found ranging from 2.75 to 5.65 kilobases (kb) and most of them occurred within the melanin (mel) gene of pIJ702, resulting in the loss of part of the mel gene sequence plus the insert. The removal of the pUC12 sequence from pWCL1 eliminated the instability. However, pUC12 alone inserted in either orientation on pIJ702 also caused the deletion in S. lividans 1326. The results indicated that the structural instability of hybrid plasmid of pIJ702 depended on the interaction between the mel sequence and the inserted sequence.

Original languageEnglish
Pages (from-to)372-378
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume140
Issue number1
DOIs
StatePublished - 15 Oct 1986

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