Spatiotemporal characterization of phagocytic NADPH oxidase and oxidative destruction of intraphagosomal organisms in vivo using autofluorescence imaging and raman microspectroscopy

Wei Tien Chang, Yi Cyun Yang, Hsueh H. Lu, I. L. Li, Ian Liau*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

(Figure Presented) The ability to generate antimicrobial reactive-oxygen species (ROS) by reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is critical for the host to defend against invading microbes. We have demonstrated the application of confocal autofluorescence microscopy and Raman microspectroscopy to characterize dynamically the phagocytic process of living macrophages in a label-free manner. In particular, we visualized the translocation of NADPH oxidases in living macrophages that are undergoing phagocytosis of invading yeasts and monitored dynamically the change at the molecular level of single intraphagosomal yeasts caused by phagocytic ROS.

Original languageEnglish
Pages (from-to)1744-1745
Number of pages2
JournalJournal of the American Chemical Society
Volume132
Issue number6
DOIs
StatePublished - 1 Mar 2010

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