Spatial orientation mapping of fibers using polarization-sensitive second harmonic generation microscopy

Vladimir A. Hovhannisyan, Po-Sheng Hu, Hsing Yuan Tan, Shean-Jen Chen*, Chen Yuan Dong

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

In this work, we present a non-invasive approach to determine azimuth and elevation angles of collagen fibers capable of generating second harmonic signal. The azimuth angle was determined using the minimum of second harmonic generation (SHG) signal while rotating the plane of polarization of excitation light. The elevation angle was estimated from the ratio of the minimal SHG intensity to the intensity when laser polarization and fiber directions were parallel to each other using experimentally determined calibration curve. Pixel-resolution images of collagen fiber spatial orientation in tendon from bovine leg, chicken leg, and chicken skin were acquired using our approach of SHG polarization-resolved microscopy.

Original languageEnglish
Pages (from-to)768-776
Number of pages9
JournalJournal of Biophotonics
Volume5
Issue number10
DOIs
StatePublished - 1 Oct 2012

Keywords

  • Collagen
  • Fiber orientation
  • Polarization-resolved microscopy
  • Second harmonic generation
  • Three-dimensional image processing

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