@inproceedings{6520b90697db44e0978b4ae8785ee226,
title = "Smart bombing a single targeted cell with femtogram order reagents using laser-induced Shockwave technique",
abstract = "Injection and delivery of small amount reagent in aqueous solution for cell chip was performed utilizing regeneratively amplified femtosecond laser system. In our new trial, the reagent integrated on a solid strip are released and delivered to targeted cells with the femutosecond laser-induced impulsive-force. The reagent was fixed in polyvinyl alcohol) or polystyrene film on a glass-substrate strip. When a single pulsed femtosecond laser was focused in the solution, the film near the focal point was fragmented and the reagent was dispersed in 45-μm φ area at 50 μm from the surface of the reagent strip. As examples cardiomyocyte beating cells of P19CL6 were bombed with epinephrine and acetylcholine, and as a result the beating ratio of the cells were quickly stimulated and suppressed, respectively. The results demonstrate that the present method is a promising key nano/micro technology for diagnosis and drug discovery.",
keywords = "Acetylcholine, Cardiomyocytes, Cell chip, Cell modification, Drug discovery, Epinephrine, Femtosecond laser, Fluorescent nanoparticles, Laser-induced impulse, P19CL6",
author = "Kazunori Okano and Noriko Takizawa and Takayuki Uwada and Yoichiroh Hosokawa and Hiroshi Masuhara",
year = "2008",
month = apr,
day = "21",
doi = "10.1117/12.762613",
language = "English",
isbn = "9780819470294",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Optical Interactions with Tissue and Cells XIX",
note = "null ; Conference date: 21-01-2008 Through 23-01-2008",
}