Smart bombing a single targeted cell with femtogram order reagents using laser-induced Shockwave technique

Kazunori Okano*, Noriko Takizawa, Takayuki Uwada, Yoichiroh Hosokawa, Hiroshi Masuhara

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Injection and delivery of small amount reagent in aqueous solution for cell chip was performed utilizing regeneratively amplified femtosecond laser system. In our new trial, the reagent integrated on a solid strip are released and delivered to targeted cells with the femutosecond laser-induced impulsive-force. The reagent was fixed in polyvinyl alcohol) or polystyrene film on a glass-substrate strip. When a single pulsed femtosecond laser was focused in the solution, the film near the focal point was fragmented and the reagent was dispersed in 45-μm φ area at 50 μm from the surface of the reagent strip. As examples cardiomyocyte beating cells of P19CL6 were bombed with epinephrine and acetylcholine, and as a result the beating ratio of the cells were quickly stimulated and suppressed, respectively. The results demonstrate that the present method is a promising key nano/micro technology for diagnosis and drug discovery.

Original languageEnglish
Title of host publicationOptical Interactions with Tissue and Cells XIX
DOIs
StatePublished - 21 Apr 2008
EventOptical Interactions with Tissue and Cells XIX - San Jose, CA, United States
Duration: 21 Jan 200823 Jan 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6854
ISSN (Print)1605-7422

Conference

ConferenceOptical Interactions with Tissue and Cells XIX
CountryUnited States
CitySan Jose, CA
Period21/01/0823/01/08

Keywords

  • Acetylcholine
  • Cardiomyocytes
  • Cell chip
  • Cell modification
  • Drug discovery
  • Epinephrine
  • Femtosecond laser
  • Fluorescent nanoparticles
  • Laser-induced impulse
  • P19CL6

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