Signal enhancement by particle concentration using dielectrophoresis in an electrowetting-driven droplet

Tsung Min Chuo, Po Huai Shih, Wen-Syang Hsu, Cheng Yeh Huang, Yu Shan Huang, Yu Tong Chen, Shih Kan Fan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

This paper describes a new method for enhancing the fluorescence signal of a bead-based immunosensor in a digital microfluidic system by dielectrophoresis (DEP) force. A single droplet containing scattered polystyrene microbeads conjugated with fluorescent dye is first moved by electrowetting to 3-phase spiral electrodes. The fluorescent microbeads are then concentrated by DEP force at 145 VRMS over 100 kHz. It is shown that the proposed microfluidic chip can evidently enhance the fluorescent intensity even there are only very few fluorescent microbeads (10 to 100), where the droplet volume is only 6.25 nanoliter. The total operation time of the concentrating process is less than 3 minutes. The proposed signal enhancement method holds great potential for bead-based immunoassays to have better limit of detection with short analysis time and small sample volume.

Original languageEnglish
Title of host publicationNANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering
Pages56-60
Number of pages5
DOIs
StatePublished - 1 Dec 2012
Event6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012 - Bangkok, Thailand
Duration: 4 Nov 20127 Nov 2012

Publication series

NameIEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
ISSN (Print)2159-6964
ISSN (Electronic)2159-6972

Conference

Conference6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012
CountryThailand
CityBangkok
Period4/11/127/11/12

Keywords

  • DEP
  • Fluorescence
  • Immunoassays
  • Microbeads
  • Microfluidics

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    Chuo, T. M., Shih, P. H., Hsu, W-S., Huang, C. Y., Huang, Y. S., Chen, Y. T., & Fan, S. K. (2012). Signal enhancement by particle concentration using dielectrophoresis in an electrowetting-driven droplet. In NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering (pp. 56-60). [6509119] (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED). https://doi.org/10.1109/NANOMED.2012.6509119