Separation of dendritic and T cells using electrowetting and dielectrophoresis

Chang An Chen, Chiun-Hsun Chen, Amir M. Ghaemmaghami, Shih Kang Fan*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

The research of immune cells is fundamental to many biological studies. Dendritic cells have the ability to induce a primary immune response in resting naive T cells. The aim of this work is to separate the activated T cells from dendritic cells on a digital microfluidic device where droplets are driven by electrowetting-on-dielectric (EWOD). The cells suspended in EWOD-driven droplets are separated and concentrated using a high frequency electric signal which generates non-uniform electric fields and dielectrophoresis (DEP) forces exerting on dendritic cells and T cells. Separation and concentration of dendritic cells and T cells are demonstrated in a droplet using EWOD and DEP.

Original languageEnglish
Title of host publication2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
Pages183-186
Number of pages4
DOIs
StatePublished - 1 Jun 2012
Event7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012 - Kyoto, Japan
Duration: 5 Mar 20128 Mar 2012

Publication series

Name2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012

Conference

Conference7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
CountryJapan
CityKyoto
Period5/03/128/03/12

Keywords

  • T cell
  • dendritic cell
  • dielectrophoresis
  • electrowetting-on-dielectric

Fingerprint Dive into the research topics of 'Separation of dendritic and T cells using electrowetting and dielectrophoresis'. Together they form a unique fingerprint.

  • Cite this

    Chen, C. A., Chen, C-H., Ghaemmaghami, A. M., & Fan, S. K. (2012). Separation of dendritic and T cells using electrowetting and dielectrophoresis. In 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012 (pp. 183-186). [6196752] (2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012). https://doi.org/10.1109/NEMS.2012.6196752