Sensitivity evaluation of rhodamine B hydrazide towards nitric oxide and its application for macrophage cells imaging

Chi Ming Wu, Yen Hao Chen, Kasala Dayananda, Tsun Wei Shiue, Chen Hsiung Hung, Wen Feng Liaw, Po Yu Chen, Yun-Ming Wang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

A colorless and non-fluorescent rhodamine derivative, rhodamine B hydrazide (RH), is applied to detect nitric oxide and form fluorescent rhodamine B (RB). The reaction mechanism of RH with NO is proposed in this study. The probe shows good stability over a broad pH range (pH > 4). Furthermore, fluorescence intensity of RH displays an excellent linearity to the NO concentration and the detection limit is as low as 20. nM. A 1000-fold fluorescence turn-on from a dark background was observed. Moreover, the selectivity study indicated that the fluorescence intensity increasing in the presence of NO was significantly higher than those of other reactive oxygen/nitrogen species. In exogenously generated NO detection study, clear intracellular red fluorescence was observed in the presence of S-nitroso- N-acetyl-D,L-penicillamine (SNAP, a kind of NO releasing agent). In endogenously generated NO detection study, increasing incubation time of RH with lipopolysaccharied (LPS) pre-treated cells could obtain a highly fluorescent cell image. These cell imaging results demonstrated that RH can efficiently penetrate into Raw 264.7 cells and be used for detection of exogenously and endogenously generated nitric oxide.

Original languageEnglish
Pages (from-to)141-148
Number of pages8
JournalAnalytica Chimica Acta
Volume708
Issue number1-2
DOIs
StatePublished - 5 Dec 2011

Keywords

  • Cell imaging
  • Fluorescent probe
  • Nitric oxide
  • Rhodamine

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