Selective imaging in second-harmonic-generation microscopy by polarization manipulation

Shi Wei Chu*, Shih Peng Tai, Chi Kuang Sun, Chi Hung Lin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Second-harmonic-generation (SHG) has proved itself as an important contrast mechanism in microscopic applications. Its noninvasiveness, optical sectioning capability, and high-penetrability provide attractive features in observation of thick biological tissues. Fibrous proteins, such as myosin and collagen, are dominant SHG harmonophores in vertebrates. Due to their biophotonic crystal nature, SHGs from these proteins are known to exhibit specific polarization dependencies, reflecting local molecule arrangements. Here the authors demonstrate a scheme to distinguish SHG from myosin-based muscle fibers and intertwined collagenous perimysium through polarization selection, without complicated staining or sample/image processing required.

Original languageEnglish
Article number103903
JournalApplied Physics Letters
Issue number10
StatePublished - 2007

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