Selection of Invasive and Metastatic Subpopulations from a Human Lung Adenocarcinoma Cell Line

Yi Wen Chu, Pan Chyr Yang*, Shuenn Chen Yang, Yu Chiau Shyu, Mary J.C. Hendrix, Reen Wu, Cheng-Wen Wu Lee

*Corresponding author for this work

Research output: Contribution to journalArticle

342 Scopus citations

Abstract

To better understand the mechanism(s) underlying lung cancer invasion and metastasis, a Transwell invasion chamber was used to select progressively more invasive cancer cell populations from a clonal cell line of human lung adenocarcinoma, CL1. Five sublines with progressive invasiveness, designated CL1-1, CL1-2, CL1-3, CL1-4, and CL1-5, were obtained through this in vitro selection process. Their invasive abilities through basement membrane matrix showed a 4- to 6-fold increase over that of the parental cells. Moreover, the sublines manifested an increase in their colony-forming ability on soft agar, tumorigenicity, and metastatic potency in severe combined immunodeficiency (SCID) mice. Examining the phenotypes of the cell lines revealed increased expression of 92 kD gelatinase and an increase in the cell population stained with anti-keratin-8 and -18 antibodies. Clonal isolation of anti-keratin-18-antibody-positive and -negative cell populations demonstrated a correlated enhancement of the invasiveness of these cells and their expression of keratin-18. These results support the notion that the metastatic behavior of lung cancer cells can be characterized with this in vitro system, and that the properties of these progressively invasive cancer cells can be clonally studied.

Original languageEnglish
Pages (from-to)353-360
Number of pages8
JournalAmerican journal of respiratory cell and molecular biology
Volume17
Issue number3
DOIs
StatePublished - 1 Jan 1997

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