Second-harmonic generation microscopy imaging of corneal superstructure

Sheng Lin Lee, Po-Sheng Hu, Yang Fang Chen, Chen Yuan Dong*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Optical clarity and mechanical strength are properties of cornea that are dictated by the microscopic superstructure of collagen lamellae. In this study, we used second harmonic generation microscopy to characterize and study chicken cornea model in thee -dimensions. Through the use of a combination of second harmonic generation (SHG) microcopy and Fouriertransform image analysis, adult chicken corneas were imaged. We found that adjacent lamellae of anterior chicken cornea stroma rotate in a counterclockwise fashion with a rotational pitch of around 0.7 degree/μm. However, the rotation stops upon reaching posterior stroma. Our approach can be extended into understanding structural architecture of human cornea and contribute to improved engineering products for corneal transplantation.

Original languageEnglish
Title of host publicationFrontiers in Optics, FiO 2017
PublisherOSA - The Optical Society
ISBN (Electronic)9781557528209
StatePublished - 1 Jan 2017
EventFrontiers in Optics, FiO 2017 - Washington, United States
Duration: 18 Sep 201721 Sep 2017

Publication series

NameOptics InfoBase Conference Papers
VolumePart F66-FiO 2017


ConferenceFrontiers in Optics, FiO 2017
CountryUnited States

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