Sample preparation for multiple-reactant bioassays on micro-electrode-dot-array biochips

Tung Che Liang, Yun Sheng Chan, Tsung Yi Ho, Krishnendu Chakrabarty, Chen-Yi Lee

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Scopus citations

Abstract

Sample preparation, as a key procedure in many biochemical protocols, mixes various samples and/or reagents into solutions that contain the target concentrations. Digital microfluidic biochips (DMFBs) have been adopted as a platform for sample preparation because they provide automatic procedures that require less reactant consumption and reduce human-induced errors. However, traditional DMFBs only utilize the (1:1) mixing model, i.e., only two droplets of the same volume can be mixed at a time, which results in higher completion time and the wastage of valuable reactants. To overcome this limitation, a next-generation micro-electrode-dot-array (MEDA) architecture that provides flexibility of mixing multiple droplets of different volumes in a single operation was proposed. In this paper, we present a generic multiple-reactant sample preparation algorithm that exploits the novel fluidic operations on MEDA biochips. Simulated experiments show that the proposed method outperforms existing methods in terms of saving reactant cost, minimizing the number of operations, and reducing the amount of waste.

Original languageEnglish
Title of host publicationASP-DAC 2019 - 24th Asia and South Pacific Design Automation Conference
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages506-511
Number of pages6
ISBN (Electronic)9781450360074
DOIs
StatePublished - 21 Jan 2019
Event24th Asia and South Pacific Design Automation Conference, ASPDAC 2019 - Tokyo, Japan
Duration: 21 Jan 201924 Jan 2019

Publication series

NameProceedings of the Asia and South Pacific Design Automation Conference, ASP-DAC

Conference

Conference24th Asia and South Pacific Design Automation Conference, ASPDAC 2019
CountryJapan
CityTokyo
Period21/01/1924/01/19

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