Sample preparation for many-reactant bioassay on DMFBs using common dilution operation sharing

Chia Hung Liu, Hao Han Chang, Tung Che Liang, Juinn-Dar Huang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

31 Scopus citations

Abstract

Sample preparation is an essential processing step in most biochemical applications. Various reactants are mixed together to produce a solution with the target concentration. Since reactants generally take a notable part of the cost in a bioassay, their usage should be minimized whenever possible. In this paper, we propose an algorithm, CoDOS, to prepare the target solution with many reactants using common dilution operation sharing on digital microfluidic biochips (DMFBs). CoDOS first represents the given target concentration as a recipe matrix, and then identifies rectangles in the matrix, where each rectangle indicates an opportunity of dilution operation sharing for reactant minimization. Experimental results demonstrate that CoDOS can achieve up to 27% of reactant saving as compared with the bit-scanning method in single-target sample preparation. Moreover, even if CoDOS is not developed for multi-target sample preparation, it still outperforms the recent state-of-the-art algorithm, RSMA. Hence, it is convincing that CoDOS is a better alternative for many-reactant sample preparation.

Original languageEnglish
Title of host publication2013 IEEE/ACM International Conference on Computer-Aided Design, ICCAD 2013 - Digest of Technical Papers
Pages615-621
Number of pages7
DOIs
StatePublished - 1 Dec 2013
Event2013 32nd IEEE/ACM International Conference on Computer-Aided Design, ICCAD 2013 - San Jose, CA, United States
Duration: 18 Nov 201321 Nov 2013

Publication series

NameIEEE/ACM International Conference on Computer-Aided Design, Digest of Technical Papers, ICCAD
ISSN (Print)1092-3152

Conference

Conference2013 32nd IEEE/ACM International Conference on Computer-Aided Design, ICCAD 2013
CountryUnited States
CitySan Jose, CA
Period18/11/1321/11/13

Keywords

  • Biochip
  • digital microfluidic biochip (DMFB)
  • dilution
  • reactant minimization
  • sample preparation

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