Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase: A chemistry-biology interdisciplinary study of the protein's structure-function- reaction mechanism relationships

Tung-Kung Wu*, Cheng Hsiang Chang, Yuan Ting Liu, Tsai Ting Wang

*Corresponding author for this work

Research output: Contribution to journalReview article

45 Scopus citations

Abstract

The oxidosqualene cyclases (EC 5.4.99-) constitute a family of enzymes that catalyze diverse cyclization/rearrangement reactions of (3S)-2,3-oxidosqualene into a distinct array of sterols and triterpenes. The relationship between the cyclization mechanism and the enzymatic structure is extremely complex and compelling. This review covers the historical achievements of biomimetic studies and current progress in structural biology, molecular genetics, and bioinformatics studies to elucidate the mechanistic and structure-function relationships of the Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase-catalyzed cyclization/rearrangement reaction.

Original languageEnglish
Pages (from-to)302-325
Number of pages24
JournalChemical Record
Volume8
Issue number5
DOIs
StatePublished - 1 Dec 2008

Keywords

  • Biomimetic
  • Molecular evolution
  • Oxidosqualene cyclase
  • Protein engineering
  • Protein plasticity
  • Site-saturated mutagenesis

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