Riboflavin immobilized Fe 3 O 4 magnetic nanoparticles carried with n-butylidenephthalide as targeting-based anticancer agents

Ching Yi Wu, Yu-Chie Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


n-Butylidenephthalide (BP) is a potential anti-cancer drug, which can be extracted from Angelica sinensis (Danggui). Previous reports have shown the effectiveness of BP in treating cancer diseases. However, BP has no targeting capacity towards specific cancer cells. To improve treatment efficiency and reduce the dose of BP used in cancer treatment, targeting-based approaches should be developed. In the present study, we used riboflavin-5′-phosphate (RFMP) immobilized iron oxide magnetic nanoparticles (Fe 3 O 4 MNPs) as carriers for BP to treat cancer cell lines derived from liver, prostate and breast. These model cancer cells overexpress riboflavin receptors on their cell membrane and are also sensitive to BP treatment. Thus, BP-binding free RFMP on MNPs can be used as probes to target these model cells, whereas BP can be readily released on target cancer cells. Cell viability was twofold lower by using Fe 3 O 4 @RFMP MNPs immobilized with BP than that achieved by using free-form BP at a similar amount. Moreover, BP-Fe 3 O 4 @RFMP MNPs have no apparent harmful effects on non-target cells. In addition, we evaluated the level of cysteine-aspartic acid protease 3 (caspase 3) in the resultant cell lysate obtained after treatment by BP-Fe 3 O 4 @RFMP MNPs to demonstrate that apoptosis is mainly involved in the growth inhibition of target cells.

Original languageEnglish
Pages (from-to)210-220
Number of pages11
JournalArtificial Cells, Nanomedicine and Biotechnology
Issue number1
StatePublished - 4 Dec 2019


  • Angelica sinensis
  • anticancer
  • drug delivery
  • iron oxide magnetic nanoparticles
  • n-Butylidenephthalide
  • riboflavin

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