Resolution studies on counter-current chromatography using supercritical fluid carbon dioxide

Tiing Yu*, Yi Hsing Chen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Counter-current chromatography (CCC) is a form of liquid-liquid partition chromatography. It requires two immiscible solvent phases; the stationary phase is retained in the separation column, generally by centrifugal force, while the mobile phase is eluted. We recently replaced the mobile phase with supercritical fluid carbon dioxide (SF CO2). Since the solvent strength of SF CO2 can be varied by changing the temperature and pressure of the system, separation adjustments are thus more versatile. We investigated the pressure and temperature effects on resolution using water and low-carbon alcohol mixtures as the stationary phases. It was demonstrated that these special properties of SF CO2 were indeed beneficial to the optimization of separations. In addition, the phase retention ratio was examined in terms of separation resolution. The results appeared very similar to those obtained from conventional traditional CCC. This study should be helpful for the future development of SF applications in CCC.

Original languageEnglish
Pages (from-to)31-39
Number of pages9
JournalJournal of Chromatography A
Issue number1-2
StatePublished - 28 Nov 1997


  • Acetophenone
  • Benzophenone
  • Counter-current chromatography
  • Mobile phase composition
  • Naphthalene
  • Resolution

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