Removal of hydrogen sulphide by immobilized Thiobacillus sp. strain CH11 in a biofilter

Ying Chien Chung, Chih-Pin Huang*, Ching-Ping Tseng

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

52 Scopus citations


An autotropic Thiobacillus sp. CH11 was isolated from piggery wastewater containing hydrogen sulphide. The removal characteristics of hydrogen sulphide by Thiobacillus sp. CH11 were examined in the continuous system. The hydrogen sulphide removal capacity was elevated by the BDST (Bed Depth Service Time) method (physical adsorption) and an immobilized cell biofilter (biological conversion). The optimum pH to remove hydrogen sulphide ranged from 6 to 8. The average specific uptake rate of hydrogen sulphide was as 1.02 x 10 -13 mol-S cell -1 h -1 in continuous systems. The maximum removal rate and saturation constant for hydrogen sulphide were calculated to be V(m) = 30.1 mmol-S day -1 (kg-dry bead) -1 and K(s) = 1.28 μmol dm -3, respectively. A criterion to design a scale-up biofilter was also studied. The maximum inlet loading in the linear region (95% removal) was 47 mmol-S day -1 (kg-dry bead) -1. Additionally, the biofilter exhibited high efficiency (> 98.5%) in the removal of hydrogen sulphide at both low (< 0.026 mg dm -3) and high (0.078 mg dm -3) concentrations. The results suggested that the Thiobacillus sp. CH11 immobilized with Ca-alginate is a potential method for the removal of hydrogen sulphide.

Original languageEnglish
Pages (from-to)58-62
Number of pages5
JournalJournal of Chemical Technology and Biotechnology
Issue number1
StatePublished - 1 May 1997


  • Biofilter
  • Hydrogen sulphide
  • Immobilized cell
  • Thiobacillus sp.

Fingerprint Dive into the research topics of 'Removal of hydrogen sulphide by immobilized Thiobacillus sp. strain CH11 in a biofilter'. Together they form a unique fingerprint.

Cite this