Regulation of the homologous two-component systems KvgAS and KvhAS in Klebsiella pneumoniae CG43

Ching Ting Lin, Hwei-Ling Peng*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


In Klebsiella pneumoniae CG43, deletion of the sensor gene kvgS reduced the kvgAS expression in M9 medium with 0.2 mM paraquat, 0.2 mM 2,2-dihydropyridyl, or 300 mM NaCl. This result shows an autoregulatory role of KvgS and a stress-responsive expression of the two-component system (2CS). The kvgS deletion also appeared to decrease the expression of kvhAS, paralogous genes of kvgAS. Additionally, measurements of the promoter activity in kvgA- mutant revealed that KvgA is probably an activator for the expression of kvgAS and kvhAS. The subsequent electrophoretic mobility shift assay, indicating a specific binding of the recombinant KvgA to the putative promoters P kvgAS and PkvhAS, also supported an interacting regulation between the 2CSs. In PkvgAS and PkvhAS, the presence of RpoS binding elements suggested an BpoS-dependent regulation. Nevertheless, the rpoS deletion reduced the expression of kvgAS but increased that of kvhAS. Moreover, the kvgA deletion reduced the expression of katG and sodC. The over-expression of KvhA altered the susceptibility to fosfomycin and an increasing activity of UDP-N-acetylglucosamine enolpyruvyl transferase, the target protein of fosfomycin, which suggesting a regulation by KvhA. Taken together, these indicated that the two 2CSs probably belong to different regulatory circuits of the RpoS regulon.

Original languageEnglish
Pages (from-to)639-648
Number of pages10
JournalJournal of Biochemistry
Issue number5
StatePublished - 1 Nov 2006


  • Fosfomycin
  • katG
  • KvgAS
  • KvhAS
  • rpoS
  • sodC
  • Two-component system

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