Reconstitution of the early steps of gliotoxin biosynthesis in Aspergillus nidulans reveals the role of the monooxygenase GliC

Shu Lin Chang, Yi Ming Chiang, Hsu Hua Yeh, Tung-Kung Wu, Clay C.C. Wang*

*Corresponding author for this work

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The gliotoxin, a member of the epipolythiodioxopiperazine (ETP), has received considerable attention from the scientific community for its wide range of biological activity. Despite the identification of gliotoxin cluster, however, the sequence of steps in the gliotoxin biosynthesis has remained elusive. As an alternative to the gene knock-out and biochemical approaches used so far, here we report using a heterologous expression approach to determine the sequence of the early steps of gliotoxin biosynthesis in Aspergillus nidulans. We identified the GliC, a monooxygenases that involved in the second step of gliotoxin biosynthesis pathway through the catalyzing the hydroxylation at the α-position of l-Phe.

Original languageEnglish
Pages (from-to)2155-2157
Number of pages3
JournalBioorganic and Medicinal Chemistry Letters
Volume23
Issue number7
DOIs
StatePublished - 1 Apr 2013

Keywords

  • Biosynthesis
  • Gliotoxin
  • Heterologous expression

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