Realignment process of actin stress fibers in single living cells studied by focused femtosecond laser irradiation

Ryohei Yasukuni, Jean Alexis Spitz, Rachel Meallet-Renault, Takayuki Negishi, Takuji Tada, Yoichiroh Hosokawa*, Tsuyoshi Asahi, Chisa Shukunami, Yuji Hiraki, Hiroshi Masuhara

*Corresponding author for this work

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Three-dimensional dissection of a single actin stress fiber in a living cell was performed based on multi-photon absorption of a focused femtosecond laser pulse. The realignment process of an actin stress fiber was investigated after its direct cutting by a single-shot femtosecond laser pulse irradiation by high-speed transmission and fluorescence imaging methods. It was confirmed that mechanical force led by the femtosecond laser cutting propagates to entire cell through the cytockelton in a 100 μs time scale. The cut actin stress fiber was realigned in the time scale of a few tens of minutes. The dynamic analysis of the realignment induced by single-shot femtosecond laser gives new information on cell activity.

Original languageEnglish
Pages (from-to)6416-6419
Number of pages4
JournalApplied Surface Science
Volume253
Issue number15
DOIs
StatePublished - 30 May 2007

Keywords

  • Actin cytoskeleton
  • Femtosecond laser
  • Fibroblast cell
  • Fluorescence imaging
  • High speed imaging
  • Laser processing

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    Yasukuni, R., Spitz, J. A., Meallet-Renault, R., Negishi, T., Tada, T., Hosokawa, Y., Asahi, T., Shukunami, C., Hiraki, Y., & Masuhara, H. (2007). Realignment process of actin stress fibers in single living cells studied by focused femtosecond laser irradiation. Applied Surface Science, 253(15), 6416-6419. https://doi.org/10.1016/j.apsusc.2007.01.047