Real-time detection of α-thrombin binding to single-strand DNA aptamers by a highly sensitive Si-based waveguide SPR biosensor

Chi Chieh Huang*, Hsin Feng Hsu, Sz Hau Chen, Kun Yu Tsai, Yang Tung Huang, Chih-Sheng Lin, Shih Hsin Hsu

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

αIn this paper, real-time characterization of α-thrombin binding to single-strand DNA (ssDNA) aptamers by novel Si-based waveguide SPR biosensors has been investigated. The gold nanoparticles (AuNPs) modified with anti-thrombin antibodies were employed to bind with α-thrombin via strong antibody/antigen affinity for SPR signal amplification. The detection limit of 1 pM for -thrombin detection was achieved.

Original languageEnglish
Title of host publicationThird Asia Pacific Optical Sensors Conference
DOIs
StatePublished - 20 Feb 2012
Event3rd Asia Pacific Optical Sensors Conference - Sydney, NSW, Australia
Duration: 31 Jan 20123 Feb 2012

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume8351
ISSN (Print)0277-786X

Conference

Conference3rd Asia Pacific Optical Sensors Conference
CountryAustralia
CitySydney, NSW
Period31/01/123/02/12

Keywords

  • sensor
  • SPR
  • surface plasmon resonance

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