RCA combined nanoparticle-based optical detection technique for protein microarray: A novel approach

Hsin-Yun Hsu, Yi You Huang*

*Corresponding author for this work

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Developing a readily available biosensor with excellent performances is the main focus of many research groups. Recently, major breakthroughs in miniaturization of molecular analysis have produced DNA and protein microarrays. The aim of our group is to develop a sensitive technique for analyzing signals on protein microarray by applying the surface plasmon resonance (SPR) method. This new detection technique for specific molecular binding utilizes rolling circles amplification (RCA) post-signal processing method [Nat. Genet. 19 (1998) 225-232] and optical visualization by nanogold particle-labeled molecules on a micro-structured chip surface. By covalent bonding of the RCA primer to the detection antibody guarantees that the linkage between the analyte and the amplified RCA product is maintained during the assay. Experimental results show that RCA has significantly enhanced sensitivity compared to conventional methods. This combination of an easily detectable signal with chip technology should have the potential to become a successful commercial application.

Original languageEnglish
Pages (from-to)123-126
Number of pages4
JournalBiosensors and Bioelectronics
Volume20
Issue number1
DOIs
StatePublished - 30 Jul 2004

Keywords

  • Nanogold
  • Protein microarrays
  • Rolling circles amplification (RCA)

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