Ratiometric temperature sensing with semiconducting polymer dots

Fangmao Ye, Changfeng Wu, Yuhui Jin, Yang-Hsiang Chan, Xuanjun Zhang, Daniel T. Chiu

Research output: Contribution to journalArticle

277 Scopus citations

Abstract

This communication describes ultrabright single-nanoparticle ratiometric temperature sensors based on semiconducting polymer dots (Pdots). We attached the temperature sensitive dye - Rhodamine B (RhB), whose emission intensity decreases with increasing temperature - within the matrix of Pdots. The as-prepared Pdot-RhB nanoparticle showed excellent temperature sensitivity and high brightness because it took advantage of the light harvesting and amplified energy transfer capability of Pdots. More importantly, the Pdot-RhB nanoparticle showed ratiometric temperature sensing under a single wavelength excitation and has a linear temperature sensing range that matches well with the physiologically relevant temperatures. We employed Pdot-RhB for measuring intracellular temperatures in a live-cell imaging mode. The exceptional brightness of Pdot-RhB allows this nanoscale temperature sensor to be used also as a fluorescent probe for cellular imaging.

Original languageEnglish
Pages (from-to)8146-8149
Number of pages4
JournalJournal of the American Chemical Society
Volume133
Issue number21
DOIs
StatePublished - 1 Jun 2011

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