Rapid diagnosis and quantification of herpes simplex virus with a green fluorescent protein reporter system

Szu Hao Kung, Yu Chun Wang, Chi Hung Lin, Rei Lin Kuo, Wu Tse Liu*

*Corresponding author for this work

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

A genetically modified cell line (Vero-ICP10-EGFP) was constructed for detection of herpes simplex virus (HSV) by a simple, rapid and direct method. The cell line was developed by stable transfection of Vero cell with a plasmid encoding the green fluorescent protein (GFP) driven by the promoter of the HSV-2 ICP10 gene. As early as 6 h after infection with HSV, fluorescence-emitting cells can be observed under a fluorescence microscope. A single infected cell emitting fluorescence can be observed with soft agar overlay by inverted fluorescence microscopy. No induction of detectable fluorescence was seen following infections with human cytomegalovirus (HCMV), varicella zoster virus (VZV), coxsackievirus A16 and enterovirus 71. Analysis by flow cytometry also demonstrated that intensity of the triggered fluorescence is proportional to the titer of HSV inoculated. Taken together, this novel GFP reporter system could become a useful means for rapid detection and quantification of HSV in clinical specimens. (C) Elsevier Science B.V.

Original languageEnglish
Pages (from-to)205-212
Number of pages8
JournalJournal of Virological Methods
Volume90
Issue number2
DOIs
StatePublished - 2000

Keywords

  • Green fluorescent protein
  • Herpes simplex virus
  • ICP10 promoter

Fingerprint Dive into the research topics of 'Rapid diagnosis and quantification of herpes simplex virus with a green fluorescent protein reporter system'. Together they form a unique fingerprint.

Cite this