Rapid circulatiing tumor cells staining in three-dimensional microwell perfusion chip

Kuan Hung Chen, Hsin Ying Chen, Yi Hsuan Weng, Hsueh Yao Chu, Fan Gang Tseng*, Song Sheng Yang, Ju Yu Tseng, Chien Ping Lin, Chih Yung Yang, Jeng Kai Jiang, Chi Hung Lin

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Immunofluorescence is widespread method for circulating tumor cells (CTCs) detection and characterization. A conventional process of immunofluorescence staining on cells requires centrifugal force to wash residual fluorescence, which is not only time consuming but also cells harmful. To speed up the staining process, a three-dimensional (3D) microfluidic device with a porous PDMS membrane is employed in this work. Through a gentle perfusion process, this device can reduce staining process time from 1-1.5 hours down to 30 minutes and still keep cell in high viability.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages407-408
Number of pages2
ISBN (Electronic)9780979806490
StatePublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 9 Oct 201613 Oct 2016

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period9/10/1613/10/16

Keywords

  • Circulating tumor cells
  • Immunofluorescence staining
  • Three-dimensional (3D) microfluidic device

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