In this paper, we devise techniques for immobilizing and allocating a single nanodiamond on the electron beam (e -beam) patterned smart substrate. The properly designed coordination markers on the semiconductor substrate and the high throughput of the confocal microscope provide us with a convenient tool to single out a nanodiamond with a size less than 100 nm and to study Raman spectroscopy. We observe a redshift in energy and broadening in the linewidth of the s p3 bonding Raman peak when the size of the diamond is decreased from 90 to 35 nm. The observed shifts and linewidth broadening arise from the phonon-confinement effects and are in good agreement with calculations reported by [Ager Phys. Rev. B 43, 6491 (1991)] and [Yoshikawa Appl. Phys. Lett. 62, 3114 (1993)].