Quinone-Modified Mn-Doped ZnS Quantum Dots for Room-Temperature Phosphorescence Sensing of Human Cancer Cells That Overexpress NQO1

Yi Ming Sung, Srivardhan Reddy Gayam, Pei Ying Hsieh, Hsin-Yun Hsu, Wei-Guang Diau, Shu-Pao Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Early detection of cancer cells in a rapid and sensitive approach is one of the great challenges in modern clinical cancer care. This study has demonstrated the first example of a rapid, selective, and sensitive phosphorescence probe based on phosphorescence energy transfer (PET) for cancer-associated human NAD(P)H:quinone oxidoreductase isozyme 1 (NQO1). An efficient room-temperature phosphorescence NQO1 probe was constructed by using Mn-doped ZnS quantum dots (Mn:ZnS QDs) as donors and trimethylquinone propionic acids as acceptors. Phosphorescence quenching of Mn:ZnS QDs from the Mn:ZnS QDs to a covalently bonded quinone was achieved through PET. Phosphorescence of Mn:ZnS QDs was turned on by the rapid reduction-initiated removal of the quinone quencher by NQO1. This probe shows low cellular toxicity and can rapidly distinguish between NQO1-expressing and -nonexpressing cancer cell lines through phosphorescence imaging.

Original languageEnglish
Pages (from-to)25961-25969
Number of pages9
JournalACS Applied Materials and Interfaces
Volume7
Issue number46
DOIs
StatePublished - 25 Nov 2015

Keywords

  • cancer cells
  • NQO1
  • phosphorescence energy transfer
  • quantum dots
  • room temperature phosphorescence

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