Quantitative analysis of multiphoton excitation autofluorescence and second harmonic generation imaging for medical diagnosis

Wei Liang Chen, Po-Sheng Hu, Ara Ghazaryan, Shean-Jen Chen*, Tsung Hua Tsai, Chen Yuan Dong

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

29 Scopus citations

Abstract

In recent years, two-photon excitation fluorescence and second harmonic generation microscopy has become an important tool in biomedical research. The ability of two-photon microscopy to achieve optical sectioning with minimal invasiveness is particularly advantageous for biomedical diagnosis. Advances in the miniaturization of the imaging system have increased its clinical potential, together with the development of quantitative technique for the analysis of data acquired using these imaging modalities. We present a review of the quantitative analysis techniques that have been used successfully with two-photon excitation fluorescence and SHG imaging. Specifically, quantification techniques using ratiometric, morphological, and structural differences to analyze two-photon images will be discussed, and their effectiveness at evaluating dermal and corneal pathologies and cancerous tumor growth will be described.

Original languageEnglish
Pages (from-to)519-526
Number of pages8
JournalComputerized Medical Imaging and Graphics
Volume36
Issue number7
DOIs
StatePublished - 1 Oct 2012

Keywords

  • Image analysis
  • Nonlinear optics
  • Optical microscopy
  • Second harmonic generation
  • Two-photon

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