Purification, crystallization and preliminary X-ray crystallographic analysis of glycosyltransferase-1 from Bacillus cereus

Yin Cheng Hsieh, Hsi Ho Chiu, Yen Chieh Huang, Hoong Kun Fun, Chia Yu Lu, Yaw-Kuen Li*, Chun Jung Chen

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Glycosyltransferases (GTs), which are distributed widely in various organisms, including bacteria, fungi, plants and animals, play a role in synthesizing biological compounds. Glycosyltransferase-1 from Bacillus cereus (BcGT-1), which is capable of transferring glucose to small molecules such as kaempferol and quercetin, has been identified as a member of the family 1 glycosyltransferases which utilize uridine diphosphate glucose (UDP-glucose) as the sugar donor. BcGT-1 (molecular mass 45.5kDa) has been overexpressed, purified and crystallized using the hanging-drop vapour-diffusion method. According to X-ray diffraction of BcGT-1 crystals to 2.10Å resolution, the crystal belonged to space group P1, with unit-cell parameters a = 54.56, b = 84.81, c = 100.12Å, α = 78.36, β = 84.66, γ = 84.84°. Preliminary analysis indicates the presence of four BcGT-1 molecules in the asymmetric unit with a solvent content of 50.27%.

Original languageEnglish
Pages (from-to)1228-1231
Number of pages4
JournalActa Crystallographica Section:F Structural Biology Communications
Volume70
DOIs
StatePublished - 1 Sep 2014

Keywords

  • Bacillus cereus
  • glycosyltransferase

Fingerprint Dive into the research topics of 'Purification, crystallization and preliminary X-ray crystallographic analysis of glycosyltransferase-1 from Bacillus cereus'. Together they form a unique fingerprint.

  • Cite this