Purification, crystallization and preliminary X-ray crystallographic analysis of chitinase from Bacillus cereus NCTU2

Chueh Yuan Kuo, Yue Jin Wu, Yin Cheng Hsieh, Hong Hsiang Guan, Huei Ju Tsai, Yi Hung Lin, Yen Chieh Huang, Ming Yih Liu, Yaw-Kuen Li*, Chun Jung Chen

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Chitinases (EC 3.2.1.14) are found in a broad range of organisms, including bacteria, fungi and higher plants, and play different roles depending on their origin. A chitinase from Bacillus cereus NCTU2 (ChiNCTU2) capable of hydrolyzing chitin as a carbon and nitrogen nutrient has been identified as a member of the family 18 glycoside hydrolases. ChiNCTU2 of molecular weight 36 kDa has been crystallized using the hanging-drop vapour-diffusion method. According to the diffraction of chitinase crystals at 1.10 Å resolution, the crystal belongs to space group P21, with unit-cell parameters a = 50.79, b = 48.79, c = 66.87 Å, β = 99.31°. Preliminary analysis indicates there is one chitinase molecule in the asymmetric unit, with a solvent content of 43.4%.

Original languageEnglish
Pages (from-to)916-919
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume62
Issue number9
DOIs
StatePublished - 1 Sep 2006

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