Purification, crystallization and preliminary X-ray analysis of a thermostable direct haemolysin from Grimontia hollisae

Yu Kuo Wang, Sheng Cih Huang, Yi Fang Wu, Yu Ching Chen, Wen Hung Chen, Yan Ling Lin, Manoswini Nayak, Yan Ren Lin, Thomas Tien Hsiung Li, Tung-Kung Wu

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Vibrio hollisae, a halophilic species recently reclassified as Grimontia hollisae, is a causative agent of gastroenteritis and septicaemia. One important pathogenic Vibrio factor, thermostable direct haemolysin (TDH), has been purified and crystallized in two crystal forms using the vapour-diffusion method. The crystals belonged to an orthorhombic space group, with unit-cell parameters a = 104.8, b = 112.4, c = 61.3 Å and a = 122.9, b = 123.3, c = 89.8 Å. The crystals contained either four or eight molecules per asymmetric unit, with predicted solvent contents of 49.4 and 46.3% and Matthews coefficients (VM) of 2.4 and 2.3 Å3 Da-1, respectively. These crystals were suitable for structure determination, which would yield structural details related to the cytotoxicity and oligomeric structure of this pore-forming toxin.

Original languageEnglish
Pages (from-to)224-227
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume67
Issue number2
DOIs
StatePublished - 1 Feb 2011

Keywords

  • Grimontia hollisae
  • Thermostable direct haemolysin

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