Purification and characterization of a catechol 1,2-dioxygenase from a phenol degrading Candida albicans TL3

San Chin Tsai, Yaw-Kuen Li*

*Corresponding author for this work

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

A eukaryotic catechol 1,2-dioxygenase (1,2-CTD) was produced from a Candida albicans TL3 that possesses high tolerance for phenol and strong phenol degrading activity. The 1,2-CTD was purified via ammonium sulfate precipitation, Sephadex G-75 gel filtration, and HiTrap Q Sepharose column chromatography. The enzyme was purified to homogeneity and found to be a homodimer with a subunit molecular weight of 32,000. Each subunit contained one iron. The optimal temperature and pH were 25°C and 8.0, respectively. Substrate analysis showed that the purified enzyme was a type I catechol 1,2-dioxygenase. This is the first time that a 1,2-CTD from a eukaryote (Candida albicans) has been characterized. Peptide sequencing on fragments of 1,2-CTD by Edman degradation and MALDI-TOF/TOF mass analyses provided information of amino acid sequences for BLAST analysis, the outcome of the BLAST revealed that this eukaryotic 1,2-CTD has high identity with a hypothetical protein, CaO19_12036, from Candida albicans SC5314. We conclude that the hypothetical protein is 1,2-CTD.

Original languageEnglish
Pages (from-to)199-206
Number of pages8
JournalArchives of Microbiology
Volume187
Issue number3
DOIs
StatePublished - 1 Mar 2007

Keywords

  • Candida albicans TL3
  • Catechol 1,2-dioxygenase
  • MALDI-TOF mass analysis

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