Protein engineering of saccharomyces cerevisiae oxidosqualene-lanosterol cyclase into parkeol synthase

Yuan Ting Liu, Tain Chang Hu, Cheng Hsiang Chang, Wen Shiang Shie, Tung-Kung Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

A Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase mutant, ERG7T384Y/Q450H/V454I, produced parkeol but not lanosterol as the sole end product. Parkeol undergoes downstream metabolism to generate compounds 9 and 10. In vitro incubation of parkeol produced a product profile similar to that of the in vivo experiment. In summary, parkeol undergoes a metabolic pathway similar to that of cycloartenol in yeast but distinct from that of lanosterol in yeast, suggesting that two different metabolic pathways of postoxidosqualene cyclization may exist in S. cerevisiae.

Original languageEnglish
Pages (from-to)5222-5225
Number of pages4
JournalOrganic Letters
Volume14
Issue number20
DOIs
StatePublished - 19 Oct 2012

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