Protection of a rat tracheal epithelial cell line from paraquat toxicity by inhibition of glucose-6-phosphatedehydrogenase

Te Chang Lee*, Guey Jen Lai, Shih Lun Kao, I. Ching Ho, Cheng-Wen Wu Lee

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Transformed rat tracheal epithelial cells (U2) were found to be 3.6-fold more sensitive than lung fibroblasts (RLF) to paraquat. Although the toxic effects of paraquat are associated with the generation of very active Superoxides, U2 cells contained higher levels of Superoxide dismutase and catalase than RLF cells. On the other hand, the specific activities of both NADPH-cytochrome c reductase and glucose-6-phosphate dehydrogenase (G6DP) were 3- to 4-fold higher in U2 cells than in RLF cells. Treatment with dehydroepiandrosterone (DHEA) and epiandrosterone (EPI), G6PD inhibitors, significantly decreased the intracellular NADPH and protected U2 cells from paraquat toxicity. Since DHEA and EPI treatment did not affect the uptake of paraquat, our results suggest that paraquat sensitivity may depend on the redox cycling-associated activities of paraquat.

Original languageEnglish
Pages (from-to)1143-1147
Number of pages5
JournalBiochemical Pharmacology
Volume45
Issue number5
DOIs
StatePublished - 9 Mar 1993

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