Programmable protein crystallization in metered encapsulated droplets

Y. W. Hsu, Chiun-Hsun Chen, S. K. Fan*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We report for the first time programmable protein crystallization based on tunable evaporation and dissolution of salt and protein precipitants of an aqueous core droplet encapsulated in an oil shell. The evaporation depended on the precisely metered aqueous and oil droplet volumes and the position of the aqueous core droplet. The dissolution of the precipitants was achieved by adjusting the temperature of the aqueous core droplet. Lysozyme protein crystal was obtained within 80 min through (1) rapid salt/protein precipitation by fast evaporation, (2) salt dissolution by heating to enhance protein crystallization, and (3) protein crystallization from the protein precipitants (seeds) by slow evaporation.

Original languageEnglish
Title of host publication2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Pages1093-1096
Number of pages4
DOIs
StatePublished - 7 May 2012
Event2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France
Duration: 29 Jan 20122 Feb 2012

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Conference

Conference2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
CountryFrance
CityParis
Period29/01/122/02/12

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