Probing lectin and sperm with carbohydrate-modified quantum dots

Anandakathir Robinson, Jim Min Fang*, Pi Tai Chou, Kuang-Wen Liao, Rea Min Chu, Shyh Jye Lee

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

79 Scopus citations

Abstract

We report the encapsulation of quantum dots with biologically important β-N-acetylglucosamine (GlcNAc) in different ratios, together with studies of their specific/sensitive multivalent interactions with lectins and sperm by fluorimetry, transmission electron microscopy, dynamic light scattering microscopy, confocal imaging techniques, and flow cytometry. These GlcNAc-encapsulated quantum dots (QDGLNs) specifically bind to wheat germ agglutinin, and cause fluorescence quenching and aggregation. Further studies of QDGLNs and the mannose-encapsulated QDs (QDMANs) with sperm revealed site-specific interactions, in which QDGLNs bind to the head of the sperm, while QDMANs spread over the whole sperm body.

Original languageEnglish
Pages (from-to)1899-1905
Number of pages7
JournalChemBioChem
Volume6
Issue number10
DOIs
StatePublished - 1 Oct 2005

Keywords

  • Biosensors
  • Fluorescent probes
  • Glycosides
  • Molecular recognition
  • Nanotechnology

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