A novel pressure transmitter for assisting local pressure sensing in a microfluidic device is proposed aiming to improve on-chip single cell evaluation. By integrating the pressure transmitter into an existing pressure sensing technique, the local pressure in a microchannel can be directly determined. The pressure transmitter transmits the pressure from one microfluidic circuit to another without fluid exchange between the two circuits. Four different kinds of transmitter designs are discussed in this work, and they are simulated using finite element analysis, and the most efficient one is further examined in experiments. The results show that the transmitter effectively transmit the pressure, and the pressure at the microchannel is successfully represented by microbeads patterns.