Preferential induction of transforming growth factor-β production in gastric epithelial cells and monocytes by Helicobacter pylori soluble proteins

Ming Shiang Wu, Jaw Town Lin, Ping Ning Hsu, Ching Yi Lin, Yuan Ting Hsieh, Yi Han Chiu, Po Ren Hsueh, Kuang-Wen Liao*

*Corresponding author for this work

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Background. The cytokines induced by Helicobacter pylori, as well as the intricate balance of proinflammatory and anti-inflammatory cytokines, are relevant to the outcomes of H. pylori infection. Transforming growth factor (TGF)-β and interleukin (IL)-10 are 2 vital anti-inflammatory cytokines that regulate mucosal immunity in various inflammatory and infectious diseases. Methods. To elucidate whether host-bacteria interaction can influence TGF-β and IL-10 production, we investigated the expression of TGF-β and IL-10 in various mammalian cell lines preincubated with H. pylori and other enteric bacteria. Results. The amount of TGF-β protein, but not IL-10, was significantly increased after stimulation with H. pylori, but other enteric bacteria did not induce TGF-β production. Different H. pylori strains isolated from patients with gastritis, peptic ulcer, gastric cancer and strains with cagA or vacA isogenic mutations showed similar effects on TGF-β induction, indicating that this effect was a constitutional characteristic of H. pylori and independent of cagA and vacA status. Conclusion. The results imply the presence of a protein factor (termed "TGF-β-inducing protein") that induces production of TGF-β. In view of the multiple effects of TGF-β, we conclude the TGF-β-inducing protein of H. pylori might mediate the immune response and contribute to the pathogenesis of H. pylori infection.

Original languageEnglish
Pages (from-to)1386-1393
Number of pages8
JournalJournal of Infectious Diseases
Volume196
Issue number9
DOIs
StatePublished - 1 Nov 2007

Fingerprint Dive into the research topics of 'Preferential induction of transforming growth factor-β production in gastric epithelial cells and monocytes by Helicobacter pylori soluble proteins'. Together they form a unique fingerprint.

  • Cite this