PMMA-based capillary electrophoresis electrochemical detection microchip fabrication

Ray-Hua Horng*, Pin Han, Hung Yu Chen, Kuan Wen Lin, Tung Mung Tsai, Jyh Myng Zen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


In this paper, a 50 μm (depth) × 50 μm (width) microfluidic channel is made on a poly(methyl methacrylate) (PMMA) substrate using thick photoresist. Openings were drilled for buffer reservoirs on an additional piece of PMMA. A final PMMA/patterned photoresist/PMMA sandwich configuration was completed using a bonding process. The thick photoresist was used as the adhesion layer and also as the microfluidic system. Using screen-printed technology for carbon and silver electrode fabrication, the microchip electrophoretic device functions were demonstrated. Successful detection of uric acid and L-ascorbic acid (the main components in human urine) validates the functionality of the proposed system. Successful ascorbic and uric acid separation in a sample from a urine donor who had consumed 500 mg of vitamins verified the proposed biochip.

Original languageEnglish
Pages (from-to)6-10
Number of pages5
JournalJournal of Micromechanics and Microengineering
Issue number1
StatePublished - 1 Jan 2005

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